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KMID : 0604520120380010015
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Journal of the Society of Cosmetic Scientists of Korea
2012 Volume.38 No. 1 p.15 ~ p.31
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Cellular Protective Effect and Liposome Formulation for Enhanced Transdermal Delivery of Persicaria hydropiper L. Extract
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Kim Jung-Eun
Lee Hye-Jin
Lim Myoung-Sun
Park Min-A
Park Soo-Nam
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Abstract
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In our previous studies, the antioxidant, anti-aging, and antibacterial activities of Persicaria hydropipier L. extract, and the moisturizing effect of cream containing P. hydropipier extract were investigated. In this study, the cellular protective effects of P. hydropipier extract and isoquercitrin, main component from P. hydropipier in 1O2-induced photohemolysis of human erythrocytes and ultraviolet B (UVB)-exposed HaCaT cells were investigated. Liposomes such as ethosome and elastic liposome for enhanced transdermal delivery were prepared. Size, loading efficiency, stability, and cumulative permeated amounts of ethosomes and elastic liposomes were evaluated. P. hydropipier extract and isoquercitrin showed more prominent cellular protective effect than (+)-α-tocopherol, known as lipid antioxidant at 5 μg/mL. P. hydropipier extract didn't show any characteristics of cytotoxicity at 50 μg/mL. When HaCaT cells were exposed to a single large dose (400 mJ/cm2) of UVB, the extract protected the cells against UVB radiation in a concentration dependent manner (12.5 ~ 50 μg/mL). Cell viability of HaCaT cells exposed to UVB 400 mJ/cm2 was increased by treatment with P. hydropipier extract or isoquercitrin from 36 % (cell viability of positve control groups) to 90 % (cell viability of P. hydropipier extract or isoquercitrin- treated groups). The size of 0.04 % P. hydropiper extract loaded ethosomes was 173.0 nm and the loading efficiency was 55.58 %. 0.04 % P. hydropiper extract loaded ethosomes were stable with as monodisperse particles for 1 week. The ethosome exhibited more skin permeability than general liposome and ethanol solution. The optimal ratio of lipid to surfactant (Tego® care 450) of 0.1 % P. hydropiper extract loaded elastic liposomes was observed to be 95 : 5. Vesicle size of 0.1 % P. hydropiper extract loaded elastic liposome was 176.5 nm. The deformability index of the elastic liposome was 16.4. The loading efficiency was 68.8 %. The elastic liposome containing P. hydropiper extract showed more skin permeability than liposome without surfactant (Tego® care 450).
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KEYWORD
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Persicaria hydropipier L., antioxidant, ultraviolet radiation, ethosome, elastic liposome
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